The one certainty in photography is that the closer we get to our subjects, the shallower our depth of field becomes. If we’re shooting with a macro lens, we have the option to stop our lens all the way down to increase the depth of field. Sometimes this can be enough to give us what we need. And sometimes it can’t.
If you’re shooting down a microscope, though, then changing the aperture isn’t really an option. So you have to get a little creative. And this is where focus stacking comes in. After recently switching up to DSLRs for microscope photography, The Thought Emporium YouTube channel decided to put this video together on their microscope photography focus stacking technique.